This important study reveals a role for IκBα in the regulation of embryonic stem cell pluripotency. The solid data in mouse embryonic stem cells include separation of function mutations in IκBα to ...

The membranes were blocked with 20 mmol/L Tris–HCl, pH 7.4, containing 150 mmol/L NaCl, 0.1% Tris‐buffered saline‐Tween and 5% milk or 5% BSA and then probed with primary antibodies diluted in ...

The sections were blocked with 5% normal donkey serum (NDS) in Tris-buffered saline with 1% Tween-20 (TBST) for 1 hour, followed by overnight incubation at 4°C with primary antibodies diluted in the ...

Transfer the proteins onto a PVDF (Polyvinylidene Fluoride) membrane. Block the membrane with 5% non-fat milk in Tris Buffered Saline with Tween 20 (TBST) at room temperature. Incubate with primary ...

Membranes were blocked in 5% skim milk powder (Sigma-Aldrich) in tris-buffered saline (TBS) with 0.1% Tween-20 for 1 hour at RT and incubated overnight at 4°C with an LPL antibody (5D2; 1:5000) in TBS ...

Afterward, tris-buffered saline containing Tween-20 (TBST; ZHONGHUIHECAI, Shanxi, China) was used to wash the PVDF membrane. The membrane was incubated overnight by rabbit mAb primary antibodies used ...

Blots were blocked with 5% milk in Tris Buffered Saline-Tween 20 and the following primary antibodies were applied: anti-p53 (D2H90, 1:1000, Cell Signaling Technology) and GAPHD (ABS16, 1:1000, Merck ...

The non-specific binding sites of the membranes were blocked using 5% BSA or 5% non-fat milk solution, which were incubated overnight (4 °C) with the primary antibody (diluted in Tris-buffered saline ...

Slides were then washed 3 times with 0.05% tris-buffered saline (TBS)-Tween, blocked in 1% BSA and 5% normal goat serum in TBS for 1 hour at room temperature, and then incubated overnight at 4°C with ...

Department of Biological Science and Technology, Center for Intelligent Drug Systems and Smart Bio-devices (IDS 2 B), National Yang Ming Chiao Tung University, Hsinchu 300, Taiwan Department of ...