Transfer the proteins onto a PVDF (Polyvinylidene Fluoride) membrane. Block the membrane with 5% non-fat milk in Tris Buffered Saline with Tween 20 (TBST) at room temperature. Incubate with primary ...

The membranes were blocked with 20 mmol/L Tris–HCl, pH 7.4, containing 150 mmol/L NaCl, 0.1% Tris‐buffered saline‐Tween and 5% milk or 5% BSA and then probed with primary antibodies diluted in ...