Ion chromatography has advanced through the accumulation of knowledge over a course of many years. Starting … See more

Ion-exchange chromatography separates molecules based on their respective charged groups. Ion-exchange chromatography retains analyte molecules on the column based on coulombic (ionic) interactions… See more

Before ion-exchange chromatography can be initiated, it must be equilibrated. The stationary phase must be equilibrated to certain requirements that depend on the experiment that you are working with. Once equilibrated… See more

A "strong" ion exchanger will not lose the charge on its matrix once the column is equilibrated and so a wide range of pH buffers can be used. "Weak" ion exchangers have a range of pH values in which they will maintain the… See more

A sample is introduced, either manually or with an autosampler, into a sample loop of known volume. A buffered aqueous solution known as the mobile phase carries the sample from the loop onto a column that contains so… See more